High moisture extrusion processing of hemp protein ingredients as influenced by their composition and physicochemical properties

The aim of this study was to investigate the influence nutrient composition, protein profile and physicochemical properties two commercial hemp ingredients, i.e., a isolate (HPI) concentrate (HPC) obtained by wet extraction oil pressing, respectively, on their processability under high moisture extrusion (HME) conditions. ingredients were processed both individually in combination with soy (SPC) pea (PPC) concentrates.HPC displayed highest concentration cysteine (1.6 g/100 g protein) free thiols (15 nmol/mg among investigated, while UPLC-MS/MS analysis highlighted higher relative 11S globulin (edestin) HPI than HPC.The low solubility water across pH range 3–8 (5–9%) hydration capacity closer that wheat gluten SPC PPC.HME trials performed using bench scale twin-screw extruder. not processable regardless conditions tested, which could be explained edestin macromolecular complexes hindered formation desired fibrous texture. Conversely, HPC provided extrudates promising textural when or PPC. peak force required cut throughout entire thickness, as determined texture analyzer equipped light knife blade probe guillotine, increased an increase for blends either PPC, is importance covalent bonds structure formation.The outcomes should confirmed at larger assess potential derived used development meat fish analogues.